In Gel Electrophoresis, why does volume of your control have to be the same as your test solution?

Answer 1

Because the effect is dependent on the volume of the gel.

The effect of electric charge on particles across a specific volume of liquid and pore size (the gel) is known as gel electrophoresis. The effective electrical field gradient will differ if the dimensions of the control are different, rendering the "control" ineffective as a control system.

This is an example of an agarose gel electrophoresis: https://tutor.hix.ai

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Answer 2

Nicholas Ahn

In gel electrophoresis, ensuring that the volume of the control is the same as the test solution is important for accurate comparison of results. This helps maintain consistency in the migration rate of molecules through the gel, allowing for reliable comparisons of band positions and intensities between samples.

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Answer from HIX Tutor

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.