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Why is ribonuclease often included in lysis buffer when extracting dna? What is its function?

Answer 1

This is an enzyme which will break down RNA, which helps to produce a lysate with DNA without RNA impurities.

The "ase" at the end of "ribonuclease" is a dead giveaway that you are working with an enzyme.

The ribonucleic acid, or RNA, is broken down by the enzyme.

Purifying the DNA as much as possible is the aim of extraction; ribonuclease aids in the removal of unwanted RNA from the lysate, which is the contents of an open cell that has been broken.

I hope this is useful.

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Answer 2

Jeremiah Allen

Ribonuclease is included in lysis buffer during DNA extraction to degrade RNA molecules. Its function is to prevent RNA contamination in the DNA sample, ensuring the purity of the extracted DNA for downstream applications.

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