What is the role of the primers in PCR?
Ivy AcostaDNA polymerase will now add the nucleotides in continuation to primer. DNA polymerase cannot simply add the nucleotides based on complementarity; instead, it requires the assistance of RNA primer. RNA primer is a short oligonucleotide strand that is complementary to our gene of interest, so it will form pairing with it.
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Lydia AdamsPrimers in PCR facilitate DNA replication by providing a starting point for DNA synthesis by DNA polymerase. They define the region to be amplified and initiate the synthesis of complementary strands.
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When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
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