What is the role of the enzyme ligase in replication?

Answer 1

Ligase fills in missing nucleotides on the lagging stand of DNA during replication.

Ligase catalises the addition of the appropriate nucleotides to fill in the gaps between the Okazaki fragments, completing the new double helix. Since the leading stand in DNA is read and added to in the same direction as the Helicase complex is moving, there are no gaps. However, the lagging stand is developed in the opposite direction, meaning replication on this side has to start and stop, leaving gaps between the finished DNA (Okazaki Fragments).

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Answer 2

Luna Cruz

The role of the enzyme ligase in replication is to catalyze the formation of phosphodiester bonds between adjacent nucleotides in the DNA strand, sealing any nicks or gaps that occur during the replication process. This helps to ensure the integrity and continuity of the newly synthesized DNA strand.

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Answer from HIX Tutor

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.