You are given a #"5-mL"# cell suspension with a cell count of #6.5 * 10^5# #"cell/mL"#. You are required to culture the cells at the density of #6.5 * 10^3# #"cell/mL"#. What dilution must you perform to obtain the required density?
Moreover, the ratio that exists between the volume of the diluted solution and the volume of the stock solution is also equal to the dilution factor.
In your case, the ratio between the two concentrations--stock to diluted-- is
This implies that the volume of the diluted solution will be equal to
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To obtain the required cell density of 6.5 * 10^3 cell/mL, you need to dilute the cell suspension. Since the initial cell density is 6.5 * 10^5 cell/mL, the dilution factor can be calculated by dividing the initial cell density by the desired cell density:
Dilution factor = (Initial cell density) / (Desired cell density)
Dilution factor = (6.5 * 10^5 cell/mL) / (6.5 * 10^3 cell/mL)
Dilution factor = 100
This means you need to dilute the cell suspension by a factor of 100.
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When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
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