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Why do bacterial transformations fail at times?

Answer 1

Lots of reasons
I'll list a few below

I'll just call it the "heat shock" process. - Maybe all of your bacteria perished because you left them in the water for too long. - No bacteria adapted the bacterial resistance plasmid. - Your plasmid was linear because your ligation reaction failed. -You over-amplicated the plates.

Many possible issues with reagents, enzymes, and reaction conditions.

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Answer 2

Sebastian Alvarado

Bacterial transformations can fail due to several reasons, including inefficient uptake of foreign DNA, degradation of the DNA by nucleases, poor cell viability, improper conditions during the transformation process (such as temperature or pH), or presence of substances inhibiting transformation (such as divalent cations).

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Answer from HIX Tutor

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.