Which of the following oligonucleotides would have the highest melting point when paired with the proper complementary strand?
A. 5’-AAAAAAAA-3’
B. 5’- ATGCATGC-3’
C. 5’- CGCGCGCG-3’
D. 5’- TTTTGGGG-3’
E. 5’- TATATATA-3’
A. 5’-AAAAAAAA-3’
B. 5’- ATGCATGC-3’
C. 5’- CGCGCGCG-3’
D. 5’- TTTTGGGG-3’
E. 5’- TATATATA-3’
Strand C will have the highest melting point.
The melting point depends on the amount of H-Bonds that need to be broken in order for the two strands to be separatet. Remember that Adenin (A) forms a base pair with Thymin (T) which results in 2 H-Bonds, while Guanine (G) forms a base pair with Cytosine (C) which results in 2 H-Bonds. By counting the number of H-Bonds you can determine which strand will have the highest melting point. The more H-Bonds there are, the more energy you need to break those intermolecular interactions and separate the 2 strands.
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The oligonucleotide with the highest melting point when paired with the proper complementary strand would be the one with the highest GC content. This is because GC base pairs have three hydrogen bonds, while AT base pairs have only two. Therefore, an oligonucleotide with more GC base pairs will have a stronger bond and a higher melting point.
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When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.
When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

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