What is the primary reason that DNA gel electrophoresis is usually undertaken using an agarose gel and not an acrylamide based gel as in protein electrophoresis?

Answer 1

Both are (were?) used, actually...

Depending on what you are sequencing, an agarose gel is the best option if you are talking about large fragments. You can change the amount of agarose in the gel matrix to change the size of the pores: a higher Agar content if you are talking about small fragments, a lower amount if you are talking about big size...

Acrylamide gels are the obvious option if you're doing sequencing (such as the Sanger-developed Chainstopper method), since you'll be working with small fragments and need to separate fragments that only differ in length by one base!

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Answer 2

The primary reason DNA gel electrophoresis is usually performed using an agarose gel instead of an acrylamide gel, as in protein electrophoresis, is because agarose gels have larger pore sizes, which are better suited for separating larger DNA molecules.

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Answer from HIX Tutor

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

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