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What enzyme is required to make copies of DNA from RNA?

Answer 1

Reverse transcriptase

Reverse transcriptase is an enzyme found naturally in retroviruses that acts as the catalyst in the transcription of retroviral RNA into retroviral DNA.

However this is not normal DNA, but is a unique type known as complementary DNA (cDNA). Discovering reverse transcriptase in viruses enabled application to construct cDNA from RNA.

RNA is extracted from the cell desired, and is placed in a PCR (Polymerase chain reaction) machine. The enzyme is added along with primers and free nucleotides. Optimal enzyme activity and maximum cDNA occur at 42–48°C.

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Answer 2

Isabella Anderson

The enzyme required to make copies of DNA from RNA is reverse transcriptase.

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Answer from HIX Tutor

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.