How do you calculate serial dilutions?

Answer 1

You multiply the original concentration by the dilution factors for each dilution.

A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step.

In serial dilutions, you multiply the dilution factors for each step.

The dilution factor or the dilution is the initial volume divided by the final volume.

#DF = V_i/V_f#

For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution,

#DF = V_i/V_f# = #(1"mL")/(10"mL") = 1/10#. This is a 1:10 dilution.

Example 1

What is the dilution factor if you add 0.2 mL of a stock solution to 3.8 mL of diluent?

#V_f# = 0.2 mL + 3.8 mL = 4.0 mL

#DF = V_i/V_f# = #(0.2"mL")/(4.0"mL") = 1/20#. This is a 1:20 dilution.

Example 2

If you did the above dilution four times, what would be the final dilution factor?

Solution 2

Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. So you multiply each successive dilution by the dilution factor.

You would transfer 0.2 mL from Tube 1 to 3.8 mL of diluent in Tube 2 and mix. Then transfer 0.2 mL from Tube 2 to 3.8 mL of diluent in Tube 3 and mix. Repeat the process until you have four tubes.

The dilution factor after four dilutions is

#DF = 1/20 × 1/20 × 1/20 × 1/20 = 1/160000# = 1:160 000

If the concentration of the original stock solution was 100 µg/µL, the concentration in Tube 4 would be

100 µg/µL × #1/160000# = 6.25 × 10⁻⁴ µg/µL

Hope this helps.

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Answer 2

To calculate serial dilutions, you need to determine the desired concentration of the final solution and the dilution factor for each step. Then, you multiply the initial concentration of the solution by the dilution factor for each step to obtain the concentration of the subsequent dilution. Repeat this process for each dilution step until you reach the desired final concentration.

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Answer from HIX Tutor

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.

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