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How can you isolate nuclei from cells?

Answer 1

When we want to isolate a nucleus from a cell then we talk about the process of enucleation.
For the process you need: a microscope, a suction pipette and a glass needle

This process is mostly used with oocytes, female egg cells.

step
We use a suction pipette (B) to hold the egg cell (A) and a glass needle (C) to remove the nucleus form the cell.
step
We push the glass needle through the zona pellucida that surrounds the egg cell. On the picture the glass needle is in the process of removing the nucleus.
step #-># the result
The egg cell's nucleus (A) is outside of the egg B.

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Answer 2

Maya Anderson

Differential centrifugation, cell lysis followed by differential centrifugation, and density gradient centrifugation are a few techniques that can be used to separate nuclei from cells. These techniques entail opening the cells to allow the contents to be removed, separating the nuclei from other cellular components according to their size, density, or other characteristics, and then purifying the nuclei further if necessary.

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Answer from HIX Tutor

When evaluating a one-sided limit, you need to be careful when a quantity is approaching zero since its sign is different depending on which way it is approaching zero from. Let us look at some examples.